fc gamma receptor tetramers human c1q human fcγri fcγriiib proteins  (Thermo Fisher)

Journal: PLOS Pathogens

Article Title: Polyfunctionality and breadth of HIV-1 antibodies are associated with delayed disease progression

doi: 10.1371/journal.ppat.1012739

Figure Lengend Snippet: Polyfunctionality was assessed by calculation of a polyfunctionality score at 3 years post-seroconversion (SC). We only assessed activating FcγR (FcγRI, FcγRIIa, FcγRIIIa, FcγRIIIb) and C1q interaction, and for each Fc feature we determined for each participant if there was a response that was higher than the median response of all responders in the cohort. The resulting scores (amount of features above the responder median, between 0 and 5, per person) were plotted for the four groups based on time between SC and acquired immunodeficiency syndrome (AIDS) (less than 3 years (n = 28), between 3 and 7 years (n = 147), between 7 and 11 years (n = 66) and more than 11 years (n = 71)) with one plot for 92BR020 gp120-specific responses and one plot for 92BR020 SOSIP-specific responses. In addition, a polyfunctionality index was calculated for each group as a quantitative measure of polyfunctionality. This is a value between 0 and 100 calculated by a weighted addition of the percentage of individuals in each category based on the number of features, derived from Larsen et al . , see ). This index is shown above each bar. Polyfunctionality of antibodies against these two strains at 6 months post-SC are shown in .

Article Snippet: Human FcγRI and FcγRIIIb proteins were acquired from Invitrogen and human purified C1q protein was acquired from Complement Technologies.

Techniques: Derivative Assay

Journal: Experimental Hematology & Oncology

Article Title: Preclinical investigations and a first-in-human phase 1a trial of JS007, a novel anti-CTLA-4 antibody, in patients with advanced solid tumors

doi: 10.1186/s40164-024-00567-7

Figure Lengend Snippet: JS007 binding affinity to CTLA-4 protein and Fc receptor by Biacore

Article Snippet: The affinity of JS007 for Fc receptors was determined using Biacore (GE Healthcare Life Sciences), in which anti-His tag antibody was coupled to a CM5 chip surface, followed by capturing the His-tagged recombinant human FcγRIIIa (CD16a) V176 (Junmeng Biomedical, 20200421), FcγRIIIa (CD16a) F176 (Junmeng Biomedical, 20200421), FcγRIIa (CD32a) R167 (Sino Biological, 10734-H08C), FcγRI (CD64) (Sino Biological, 10256-H08S), and FcRn (Sino Biological, CT009-H08H).

Techniques: Binding Assay, Recombinant